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KMID : 0356420040220020075
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Journal of Korean Andrology
2004 Volume.22 No. 2 p.75 ~ p.80
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Increased Expression of Hypoxia-Inducible Factor-1α in Castrated Rat Penile Tissue
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Yang Dae-Yul
Lee Won-Ki
Kim Ki-Wook
Suh Chang-Duk
Lee Young-Goo
Kim Ha-Young
Kim Sung-Yong
Park Heung-Won
Cho Seong-Jin
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Abstract
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Purpose: Hypoxia-inducible factor 1(HIF-1) is a transcriptional activator of genes whose products are involved in systemic, local, and cellular responses to hypoxia. We investigated the effect of androgen deprivation on the expression of HIF-1α and related proteins in the penile corpus cavernosum of castrated rat.
Materials and Methods: Thirty adult male Sprague-Dawley rats(250∼350 gm) were divided into 3 groups of 10 each: sham operation(group 1), bilateral orchiectomy(group 2), and bilateral orchiectomy plus hormone replacement(group 3). Testosterone propionate(2 mg/day for 4 weeks) was used for hormone replacement. At 4 weeks after surgery, serum testosterone and erythropoietin were measured, and the expression of HIF-1α and VEGF were examined by immunohistochemical staining and Western blot of corpus cavernosum.
Results: There was no significant change in serum erythropoietin among the three groups. HIF-1α and VEGF immuno-positive cells were dense in vascular endothelium and cavernosal smooth muscle and showed more intense staining in the orchiectomy group compared with the control and sham operation groups. The amount of HIF-1α and VEGF proteins detected by Western blot were also increased in the orchiectomy group compared with the control and sham operation groups.
Conclusions: These results suggest that increased HIF-1α expression in the penile tissue of castrated rat results from adaptive responses to hypoxia, and testosterone deprivation may contribute to hypoxic injury in the cavernosal microenvironment.
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KEYWORD
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Hypogonadism, Hypoxia-inducible factor 1, Vascular endothelial growth factor, Erectile dysfunction
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